Table 1 Primer sequences used for PRMT5 and MEP50 cloning

From: MEP50/PRMT5-mediated methylation activates GLI1 in Hedgehog signalling through inhibition of ubiquitination by the ITCH/NUMB complex

Gene

Primer sequence

MEP50 (NM_001317062.1)

Forward: cggaattcATGCGGAAGGAAACCCCACCC

Reverse: gctctagaCTACTCAGTAACACTTGCAGG

PRMT5 (NM_001039619.1)

Forward: cggaattcATGCGGGGTCCGAACTGGGGG

Reverse: cggaattcCTAGAGGCCAATGGTATATGA

  1. cDNA pool derived from HeLa cells was used for MEP50 and PRMT5 cloning
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