Fig. 4

Mislocalization of integrin α6 in the epidermis of Beclin 1 cKO mice. a Representative images of integrin α6 mislocalization in the epidermis of Beclin 1 cKO mice. Frozen sections were immunostained with an anti-integrin α6 antibody (red). Nuclei are counterstained with DAPI (blue). Scale bars = 20 µm. b Dorsal skin of the indicated E18.5 embryos were lysed and analyzed using an anti-integrin α6 antibody. Actin was used as a loading control. Representative images of two independent experiments are shown. c Basal cells were isolated from the epidermis of indicated mice (Postnatal day 0 neonates), scattered into collagen-coated plastic dishes. After 3 h, cells were observed using a phase-contrast microscope. Scale bars = 500 µm. d Similar experiments to a were performed using an anti-integrin β4 antibody, instead of an anti-integrin α6 antibody. e, f Frozen sections of the epidermis of the indicated mice were immunostained with anti-transferrin receptor and anti-integrin α6 antibodies in e and with anti-EEA1 and anti-integrin α6 antibodies in f. Nuclei were counterstained with DAPI (blue). Scale bars = 10 µm. Uncropped immunoblot images are provided in Supplementary Figure 4d