Fig. 6

Treatment with vesicular trafficking inhibitor promotes integrin α6 puncta formation and disrupts differentiation in primary keratinocyte. a Primary keratinocytes were differentiated by the addition of CaCl₂ together with primaquine (500 µM). After 6 h, cells were assayed for internalized anti-integrin α6 antibody and  immunostained with anti-EEA1 antibody. Nuclei were counterstained with DAPI (blue). Representative images are shown. Scale bars = 10 µm. b Primary keratinocytes were differentiated by the addition of CaCl₂ together with primaquine (20 µM), Chloroquine (2.5 µM), and E64d (10 µM)/pepstatin (10 µM). On the indicated days, cells were lysed and analyzed for the expression of the indicated proteins by western blot analysis. Representative images of two independent experiments are shown. Uncropped immunoblot images are provided in Supplementary Figure 5b