Fig. 1
From: Oxylipins mediate cell-to-cell communication in Pseudomonas aeruginosa
10-HOME and 7,10-DiHOME induce the expression of the diol synthase operon. a In vitro bioconversion of oleic acid (OA) into 10-HOME and 7,10-DiHOME oxylipins using the periplasmic fraction isolated from PAO1. The expression of the diol synthase enzymes was dependent on culturing PAO1 in the presence of OA. b Expression of β-galactosidase (β-gal) activity in PAO1 (pDSp-lacZ) and ΔDS (pDSp-lacZ) treated with OA (1 mg mL−1). No β-gal activity was detected in PAO1 (pDSp-lacZ) in the absence of OA. OA induced at least threefold more β-gal activity in PAO1 (pDSp-lacZ) than ΔDS (pDSp-lacZ). c Expression of β-gal activity in ΔDS (DSp-lacZ) treated with OA or oxylipins at the shown concentrations. ΔDS (pDSp-lacZ) showed fivefold more β-gal activity when treated with 10-HOME or 7,10-DiHOME than with OA and the increase in β-gal expression was dose dependent. Means and s.d. are from three independent experiments. 10-HOME (10S)-hydroxy-(8E)-octadecenoic acid, 7,10-DiHOME 7S,10S-dihydroxy-(8E)-octadecenoic acid. ****Significantly different, unpaired two-tailed t-test, P < 0.0001
