Fig. 5
From: NapM enhances the survival of Mycobacterium tuberculosis under stress and in macrophages
NapM is essential for the survival of M. tuberculosis under stress. NapM was required for the survival of M. tuberculosis under H2O2 and SDS stress (a) and within macrophages during infection (b). RAW264.7 cells were infected with mutant or wild-type strains of M. tuberculosis H37Ra, and the mycobacterial survival was assayed by determining colony-forming units (CFUs) at 4, 24, and 48 h after infection. Symbols represent each biological replicate and bars indicate means ± standard errors calculated from three independent experiments. The P values of the results (<0.001) are indicated by triple asterisks (***). c, d A summary for the NapM-induced regulation of DNA replication initiation and M. tuberculosis growth. A pathway describing DnaA activities regulated by NapM (c). A model showing that NapM coupled environmental signals with the regulation of DNA replication initiation to promote either proliferative or survival mode in M. tuberculosis (d). SDS, sodium dodecyl sulfate
