Fig. 6 | Communications Biology

Fig. 6

From: 90Y-NM600 targeted radionuclide therapy induces immunologic memory in syngeneic models of T-cell Non-Hodgkin’s Lymphoma

Fig. 6

90Y-NM600 elicits a T-cell mediated immune response against EL4 tumor. a Representative 200x images of CD8 and FoxP3 immunohistochemistry staining of EL4 tumors at days 3 and 6 after 90Y-NM600 treatment. Areas of positive staining (brown color) are indicated with arrow heads (blue arrows: CD8 + ; black arrows: FoxP3 + ). Scale bar represents 100 μm. b, c Quantitative analysis of the stained slides. A significant increase (P < 0.0001) in the amount of infiltrating CD8 + T-cells was observed at day 6 post-injection of 90Y-NM600. Levels of Foxp3 + cells significantly (P < 0.0001) declined in EL4 tumors at days 3 and 6 post 90Y-NM600 treatment, compared to vehicle-treated controls. d Tumor volume at day 10 after s.c. EL4 cells implantation in naïve vs. CR mice. Naïve mice developed EL4 tumors with 100% penetrance while all CR complete rejected the tumors. e ELISA of IFN-γ release by splenocytes coincubated in vitro with EL4 cells. Splenocytes of CR mice showed significantly higher IFN-γ release (P < 0.0001) compared to irradiated controls upon stimulation with EL4 cells. Numerical data are presented as mean ± SD

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