Fig. 4

Inhibition of miR-200a leads to decreased glial cell proliferation after injury. EdU incorporation experiments revealed a homeostatic level of proliferative GFAP⁺ glial cells in the spinal cord. a Homeostatic levels of cell proliferation in uninjured spinal cord; b proliferation of GFAP⁺ cells increases 4 days post injury. c Inhibition of miR-200a results in a significant decrease in the percent of proliferative GFAP⁺ glial cells at 4 days post injury. Scale bar = 50 μm. d Quantification of the percent of EdU⁺/GFAP⁺ glial cells in uninjured, 4dpi control and 4dpi inhibitor spinal cords (n = 8). ***p ≤ 0.001. Error bars represent ± S.T.D. Scale Bar = 75 μm