Table 1 Summary of binding data obtained from kinetic analysesa

From: Wnt5a is a TLR2/4-ligand that induces tolerance in human myeloid cells

Ligand on surface

Analyte injected

Bmax (RU)

KD (nM)

TLR4

Wnt5a

226 ± 0.9

44.5 ± 2.0

Wnt3a

(147 ± 30)b

(166 ± 43)b

TLR4/MD2

Wnt5a

328 ± 1.8

35.5 ± 5.1

Wnt3a

170 ± 1.2

36.1 ± 3.0

TLR2

Wnt5a

274 ± 1.5

26.5 ± 4.5

Wnt3a

158 ± 0.1

41.0 ± 0.2

  1. aBinding curves [responses at steady-state (Req) vs. analyte concentration] were fit to a one-site hyperbola model to calculate Bmax (Response in resonance units (RU)) and KD (± % SE).
  2. bWnt3a showed an almost linear increase in binding to TLR4, explaining the poor fit
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