Fig. 7
Treatment with P31-43 induced in enterocytes a transient delay of the EGF/EGFR trafficking in controls and a prolonged delay in CD patients. EGF/EGFR trafficking after P31-43 in controls and CD biopsies. a Images of immunofluorescence staining of crypts in biopsies from patients and controls that had been cultured in medium alone for 24 h (medium) or in the presence of EGF and P31-43 for 3 or 24 h. The white color in the merge panels indicates co-localization of EGFR (magenta) and EEA1 (green). Nuclei are in blue. Representative fields. Scale bar = 20 µm. b Statistical analysis of the co-localization coefficient of EGFR with EEA1. c Images of immunofluorescence staining of villi in biopsies from patients and controls that had been cultured in medium alone for 24 h (medium) or in the presence of EGF and P31-43 for 3 or 24 h. The white color in the merge panels indicates co-localization of EGFR (magenta) and EEA1 (green). Nuclei are in blue. Representative fields. Scale bar = 20 µm. d Statistical analysis of the co-localization coefficient of EGFR with EEA1. In all experiments, five subjects from each group of patients (GCD CD and GFD CD) and controls (CTR) were examined. Three independent experiments were performed on samples from each group. Columns represent the means, and bars represent standard deviations; hatched columns indicate P31-43 treatment. One-way ANOVA Bonferroni corrected. *p < 0.05, **p < 0.01, ***p < 0.001
