Fig. 5
Polyploid states are present in freshly isolated murine c-kit+ CICs. Hematopoietic lineage negative, c-kit positive CICs were freshly isolated and sorted for viability and ploidy content from FVB adult male mice. Using the cultured FVB CSC as a tetraploid control (a), fresh isolates of Lin– ckit+ CICs exhibit a mixture of diploid and tetraploid cells (b) shown by an overlay of tetraploid control versus fresh isolate sample (c). Lin–ckit+ CICs possess a similar percentage and cell count distribution of diploid and tetraploid cells with a small fraction of cells with ploidy greater than tetraploid, statistically analyzed using one-way ANOVA (d). Summary of polyploid state from FVB mouse lin–ckit+ cells of the heart, with compiled in situ and freshly isolated cells, revealing a mixture of mononuclear diploid and tetraploid levels, while cultured CSCs are tetraploid; all groups demonstrated a small fraction of tetraploid cells. **P < 0.01; ***P < 0.001. Data are presented as mean ± SEM and analyzed using one-way (d) or two-way (e) ANOVA with Bonferroni post hoc test
