Fig. 1 | Communications Biology

Fig. 1

From: THETA system allows one-step isolation of tagged proteins through temperature-dependent protein–peptide interaction

Fig. 1

Temperature dependency of the C13 monoclonal antibody. a cCRY4 C-terminal extension (CCE) sequence and epitope locations for the C13 monoclonal antibody. b Western blot analysis of GST-cCRY4CCE using C13–C15 anti-cCRY4CCE antibodies. The first antibody reactions were performed at 4 °C or 37 °C and the 2nd were performed at 4 °C. Overall picture is shown in Supplementary Fig. 2a. c Temperature dependencies in affinity constants for C13–C15 mAbs to GST-cCRY4CCE or GST-CT9. The affinity constants were measured by indirect competitive ELISA at 4, 15, 26, and 37 °C. Error bars represent standard deviation (n = 4)

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