Fig. 3 | Communications Biology

Fig. 3

From: THETA system allows one-step isolation of tagged proteins through temperature-dependent protein–peptide interaction

Fig. 3

Thermal-elution-based purification and affinity constants of C13 mAb and CT9 fusion proteins. Various CT9 fusion proteins were purified from E. coli extract, separated by SDS-PAGE, and subjected to silver staining (overall pictures are shown in Supplementary Fig. 4). The affinity constants between the C13 mAb and each CT9 fusion protein were measured by indirect competitive ELISA at 4, 15, 26, and 37 °C. CT9 × 3-D2-GST was not used for analysis of affinity constants because of contamination with many degraded products (CT9x1-D2-GST and CT9x2-D2-GST). Error bars represent standard deviation (n = 3)

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