Fig. 5
Thermal-elution-based purification of THETAS-containing proteins and THETAL by the THETA system. a Schematic drawing for thermal-elution-based purification of THETAS-tagged protein or cCRY4 using MBP-THETAL-His-gel. b GST-D2-CT9 (THETAS) was thermo-purified from an E. coli extract from 500 mL of culture using MBP-THETAL-His-gel. c cCRY4 was purified from 5 L culture of budding yeast using MBP-THETAL-His-gel. d Schematic drawing for thermal-elution-based purification of THETAL-His using THETAS-gel. e THETAL-His was purified by the THETA system from 500 mL of E. coli culture using THETAS-gel
