Table 1 Sequence of primers using cloning and construction of C13scFv
Name | Sequence |
|---|---|
Heavy chain constant region | 5′-GTG TAC AAT AGA CAG ATG GGG GTG TCG T-3′ |
Heavy chain FR1 region (MH1) | 5′-GTC GAC SAR GTN MAG CTG SAG SAG TC-3′ |
Kappa chain constant region | 5′-GGT ACC GGA TAC AGT TGG TGC AGC ATC-3′ |
Kappa chain FR1 region | 5′-CTC GAG GAY ATT GTG MTS ACM CAR WC-3′ |
C13_VL_for | 5′-CCG GCG ATG GCC ATG GAC ATT GTG ATG ACC CAA TCT CAC A-3′ |
C13_VL_rev | 5′-GGA TAC AGT TGG TGC AGC ATC AGC C-3′ |
Linker_for | 5′-GCA CCA ACT GTA TCC GGA GGC GGT GGT TCA GGT GG-3′ |
Linker_rev | 5′-CTC CAG CTT AAC CTC AGA ACC TCC TCC GCC CGA TC-3′ |
C13_VH_for | 5′-GAG GTT AAG CTG GAG GAG TCT GGA G-3′ |
C13_VH_rev | 5′-GCT CGA ATT CGG ATC ATA GAC AGA TGG GGG TGT CGT TTT G-3′ |
In_fusion_MBP_for | 5′-CCA GCC GGC GAT GGC CCC ATG GAT GAA AAT CGA AGA AGG TAA ACT GGT AAT CTG G-3′ |
In_fusion_MBP_rev | 5′-ATC ACA ATG TCC ATG CCC CTT CCC TCG ATC CCG AGG T-3′ |
