Fig. 5
Characterization and analysis of mitochondrial subpopulations by ΔΨm isolated by FAMS. a PE+ events were gated from size and FITC+ [MTG/JC1-Green] events, indicative of high-ΔΨm mitochondria. b FCCP significantly reduced the number of high-ΔΨm mitochondria, mean ± SEM, n = 3 (P < 0.05*). c High-ΔΨm (FITC+PE+) mitochondria generated significantly more ATP than low-ΔΨm (FITC+PE−) mitochondria, and ATP production was significantly reduced after preincubation with FCCP. ND none detected; mean ± SEM, n = 3 (P < 0.01**). d High-ΔΨm and low-ΔΨm mitochondrial subpopulations were assessed for size distribution based on FSC–PMT (representative histogram for n = 3). e Each ΔΨm subpopulation was assessed for levels of mt-Nd1 and mt-Nd4 by qPCR, mean ± SEM, n = 3 (P < 0.05*, P < 0.01**). f High-ΔΨm mitochondria were further segregated into small and large populations and assessed for levels of mt-Nd1 and mt-Nd4 by qPCR, mean ± SEM, n = 3 (P < 0.0001****)
