Fig. 1

Determination of the SfEiger sequence. a Schematic overview of the workflow for the determination of the sequence and structure. b 2Fo–Fc electron density map contoured at 1.5σ after main-chain tracing with SHELXE and a single round of refinement. c Numerous side chains can be clearly identified (tyrosine on the left, tryptophan on the right) in the unbiased density maps. d Schematic representation of the three exons that code for the TNF alpha domain of SfEiger. The numbers under the boxes indicate the nucleotide positions at the beginning and at the end of each genomic sequence, respectively. Introns are indicated by angled lines