Table 2 Zinc-binding affinity and stoichiometry of WT and mutant AztD

From: Crystal structures of AztD provide mechanistic insights into direct zinc transfer between proteins

Protein

Site

Kd ± S.D. (nM) (n = 3)

WT PdAztD23

1

0.7 ± 0.3

 

2

54 ± 8

 

3

340 ± 110

ΔS1 PdAztD

2

1.3 ± 0.7

 

3

248 ± 81

ΔS2 PdAztD

1

0.1*

 

3

158 ± 35

WT PdAztC40

1

0.3 ± 0.1

  1. *Uncertainties for this value could not be estimated as the Kd appears to be below the detection limit of this assay. The data were fitted with the indicated value
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