Fig. 2

Computational framework for VH-VL pairing. a Data analysis workflow. b Cell quality filtering based on the number of reads for VH and VL assemblies and VH and VL certainty. Data shown are from one human sample, Donor 1 (1). c Read coverage for quality-filtered VH and VL assemblies for three different species. Positions within VH and VL are based on Kabat numbers, CDR regions are indicated by shaded regions. Solid lines indicate the median, dashed lines interquartile range. Data shown for rat, mouse, and human are from samples Rat 1, Mouse 1, and Donor 1 (1), respectively. d Overview of B-cell repertoire data generated for this study. Bar graphs show data for independent samples. From top to bottom, number of cells captured, percentage of cells with at least one complete VH and VL assembly, percentage of cells that pass quality filtering, number of cells that pass quality filtering