Fig. 6 | Communications Biology

Fig. 6

From: Compression-induced expression of glycolysis genes in CAFs correlates with EMT and angiogenesis gene expression in breast cancer

Fig. 6

Cancer progression is associated with the compression-induced upregulation of ENO2, HK2, or PFKFB3 gene in cancer-associated fibroblasts. a The proliferation assay (n = 3 independent experiments) and b the EMT-related gene expression analysis (n = 9 independent experiments) of the breast cancer cells treated with compressed CAF-derived CM. To block the compression-induced upregulation of ENO2, HK2, or PFKFB3 gene, CAF cells were pre-treated with c-Jun inhibitor or transfected with the shRNAs against the corresponding genes before loading compression. Gene expression was analyzed using real-time PCR. Red and blue lines mean a significant promotion and inhibition, respectively. Error bars and p-values were determined by Whiskers (Min to Max) and unpaired two-tailed t-test, respectively. CS is the abbreviation of compressive stress. Source data are provided as Supplementary Data 1

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