Table 2 Conditions tested and efficiencies of transfections

From: High-efficiency genomic editing in Epstein-Barr virus-transformed lymphoblastoid B cells using a single-stranded donor oligonucleotide strategy

Plasmida

Transfection conditionsb

Number of cells (million)

Plasmid (µg)

Plasmid (molecules)

Approximate transfection efficiency (microscopy, percent GFP + cells)

GFP

X-001

4

2

6.78E + 12

0

T-020

4

2

6.78E + 12

10–15

U-009

4

2

6.78E + 12

10–15

pCAG-eCas9-GFP-U6-gRNA

U-009

2

5

4.76E + 11

0

U-009

4

10

9.51E + 11

1

U-009

2

16.6

1.58E + 12

0

U-009

4

33.3

3.17E + 12

2–5

  1. aAddGene 79145
  2. bAmaxa Nucleofector II programs
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