Table 3 Transfection efficiency testing using fluorescence-activated cell sorting (FACS)
Total events | LCLs | Single cells (front scatter) | Single cells (side scatter) | GFP positive | % GFP positive | |
|---|---|---|---|---|---|---|
Replicate 1 | 10,000 | 7965 | 7629 | 7493 | 143 | 1.91% |
Replicate 2 | 10,000 | 7687 | 7421 | 7349 | 94 | 1.28% |
Replicate 3 | 10,000 | 7338 | 7108 | 7014 | 82 | 1.17% |
Mean | 1.45% | |||||
Standard deviation | 0.40% |