Table 3 Transfection efficiency testing using fluorescence-activated cell sorting (FACS)

From: High-efficiency genomic editing in Epstein-Barr virus-transformed lymphoblastoid B cells using a single-stranded donor oligonucleotide strategy

 

Total events

LCLs

Single cells (front scatter)

Single cells (side scatter)

GFP positive

% GFP positive

Replicate 1

10,000

7965

7629

7493

143

1.91%

Replicate 2

10,000

7687

7421

7349

94

1.28%

Replicate 3

10,000

7338

7108

7014

82

1.17%

     

Mean

1.45%

     

Standard deviation

0.40%