Fig. 1 | Communications Biology

Fig. 1

From: Proteomic profiling of extracellular vesicles allows for human breast cancer subtyping

Fig. 1

Extracellular vesicle isolation and characterization. a Cell lines used for EV isolation. b EV isolation and (phospho)proteomics workflow. c Cryo-EM images of purified MDAMB231 EVs and size distribution of the isolated vesicles determined using ImageJ software. d Comparative SDS-PAGE profile of a whole-cell lysate and an EV lysate. e Western blot of exosomal marker CD81 in the MDAMB231 EV lysate and whole-cell lysate (the full blot can be found in Supplementary Fig. 1)

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