Fig. 5
From: Mechanical strain promotes skin fibrosis through LRG-1 induction mediated by ELK1 and ERK signalling
Mechanic loading induces LRG-1 expression via FAK-ERK signaling pathway. a Expression level of phosphorylated and total FAK after HDFs were subjected to mechanical stretching (10%) over different periods of time. b Western blotting analysis of LRG-1 and phosphorylation and total FAK in HDFs subjected to mechanical stretching (10%) and treated (or not treated) with FAK inhibitor (FAK-I) PF573228. c Expression level of phosphorylated and total JNK, ERK, and p38 after HDFs were subjected to mechanical stretching (10%) over different periods of time. d Mechanic loading-induced LRG-1 expression with small molecule inhibition of JNK (SP600125), ERK (PD98059), or p38 (SB203580). e Western blotting analysis of phosphorylation and total ERK in HDFs subjected to mechanical stretching (10%) and treated (or not treated) with FAK-I. f Immunofluorescence staining for ERK and LRG-1 in HDFs subjected to mechanical stretching (10%) and treated (or not treatment) with FAK-I or ERK-I. ERK are labeled in red and LRG-1 in green. (Scale bar = 50 μm). Results were represented as means with standard errors (n = 3 independent experiments). *P < 0.05, **P < 0.01, ***P < 0.001
