Fig. 1

Structure of the ExbB-ExbD subcomplex in lipid nanodiscs. a View perpendicular from the membrane of the ExbB–ExbD cryo-EM structure represented as isosurfaces. The protein contribution is colored in gray, while the contribution of the nanodisc is colored in green. The boundary of the nanodisc allows one to estimate the position of the lipid membrane. The reconstructed atomic model is shown in ribbon representation. b Same as A but viewed from the periplasm. c Ribbon representation of the reconstructed atomic model, colored in cyan for the ExbB pentamer, red and yellow for the two TM domains of the two ExbD subunits. For clarity only three ExbB subunits are represented. The TM pore formed by the ExbB pentamer is above the membrane plane. The essential Asp25 of ExbD are shown with red circles. d Same as C, viewed from the periplasm