Fig. 4 | Communications Biology

Fig. 4

From: Early stage prion assembly involves two subpopulations with different quaternary structures and a secondary templating pathway

Fig. 4The alternative text for this image may have been generated using AI.

Quaternary structural evolution of isolated PrPSc assemblies in P1 and P2 peaks on quiescent incubation. a SV profile of PMCA products seeded with 106-diluted 127 S brain homogenate, leading to the formation of P1 and P2 assemblies (as in Fig. 2a). The fractions corresponding to P1 and P2 peaks were pooled as indicated, and further incubated for 7 days at 37 °C in quiescent conditions, prior SV analysis. b On quiescent incubation, most of the assemblies present in the pooled P1 fractions evolved and formed P2. c On quiescent incubation, the pool of P2 fractions did not evolve, underlying the irreversible character of the P1 to P2 transformation and the absence of an equilibrium between P1 and P2. The results shown are the mean ± SEM values from n = 3 independent fractionations

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