Fig. 7
From: RGS2 drives male aggression in mice via the serotonergic system
Exogenous expression of RGS2 in serotonergic neurons induces c-fos expression in the dorsal raphe nucleus (DRN) and ventrolateral part of the ventromedial nucleus hypothalamus (VMHvl). (a) Representative images of the DRN, higher magnification of boxed DRN area, VMH, and a higher magnification of the VMHvl area from controlhi, ePet-Rgs2hi, Rgs2−/−, and Rgs2−/−/ePet-Rgs2hi mice following 7 days of resident intruder tests. Asterisk represents the analyzed VMHvl region. Increased relative number of cells expressing c-fos after 7 days of resident intruder testing compared to day 0 (naive) in the DRN (b) and VMHvl (c) from controlhi (black circles), ePet-Rgs2hi (dark squares), Rgs2−/−/ePet-Rgs2hi (light squares), and Rgs2−/−(grey diamonds) mice. The number of slices analyzed is reported within parentheses for each mouse line. Mice (≥3) were tested per line. Data were reported as the average total number of c-fos-positive cells/slice within parentheses for each genotype, mean ± SEM. Statistical significance was evaluated with ANOVA (*p < 0.05, **p < 0.01, ***p < 0.001)
