Fig. 3
Structure of apo ToxTENV256. a Alignment of the crystal structure of apo ToxTENV256 (6P7T), dark gray, with the structure of UFA-bound ToxTENV256 (6PB9), light gray. Helices α2 and α3 of apo ToxTENV256 are colored green. b Close-up view of helices α2 and α3 of ToxTENV256. UFA-bound ToxTENV256 is colored light gray. Apo ToxTENV256 is colored blue to green. c Close-up view of the new helix in apo ToxTENV256 located between helix α1 and β9. UFA-bound ToxTENV256 is colored light gray. Apo ToxTENV256 is colored blue to green. d Close-up view of the new salt-bridge in apo ToxTENV256 between helices α1 and α3. UFA-bound ToxTENV256 is colored light gray, apo ToxTENV256 is colored blue to green. Amino acids of UFA-bound ToxTENV256 are labeled in black, amino acids of apo ToxTENV256 are labeled in green. e Structural alignment of the regulatory domain of apo ToxTENV256 to the AraC regulatory domain dimer. AraC is colored gray, the regulatory domain of apo ToxTENV256 is colored blue to green.
