Fig. 5 | Communications Biology

Fig. 5

From: Telomere analysis using 3D fluorescence microscopy suggests mammalian telomere clustering in hTERT-immortalized Hs68 fibroblasts

Fig. 5

Analysing telomeres in 3D using Imaris. a Example of a deconvolved, 3D image of an Hs68 cell stained with PNA TelC647-conjugated probe (magenta) visualized in the Imaris program. b The centre of the fluorescence signal (small dots) was detected based on Gaussian filtering in Imaris. This was used to determine the location of each telomere within the image. c Visualization of the fluorescence signal of two telomeres (magenta) from above (XY) and from the side (Z). Spots were assigned to the fluorescence intensity that represents telomeric PNA probe. Overlay of the spots with the fluorescence intensity from above (XY) and from the side (Z) shows how accurate the assigned spots reflect telomeric signal. d Overlay of the spots with the fluorescence intensity representing the telomeres in this 3D image of an Hs68 cell. Spots were colour-coded based on volume and visualized from above. Scale bars = 2 µm.

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