Fig. 7 | Communications Biology

Fig. 7

From: Telomere analysis using 3D fluorescence microscopy suggests mammalian telomere clustering in hTERT-immortalized Hs68 fibroblasts

Fig. 7

Hs68 + hTERT cells have longer and bigger telomeres when compared to Hs68. a Frequency distribution of the Intensity Sum of the individual spots assigned in Imaris in 3D. Intensity Sum represents telomere length of the telomeres in Hs68 and Hs68 + hTERT. b Histogram of the logarithm of the Intensity Sum of the spots. c Volume measurements of the individual spots in 3D. Telomeres in Hs68 + hTERT cells are on average twofold bigger when compared to telomeres in Hs68 cells. d, e The coefficient of determination, R2, demonstrates a non-linear correlation between intensity and volume of individual spots in Hs68 and Hs68 + hTERT. A weaker R2 is observed in Hs68 + hTERT cells compared to Hs68. Note the difference in X and Y axes when comparing Hs68 (d) with Hs68 + hTERT (e). f Telomere count in 2D using the Find Maxima setting in ImageJ and various noise tolerances. g Telomere count in 3D using Imaris ‘Spots’ feature. Hs68 + hTERT cells have on average less telomeres per cell compared to Hs68. Two-tailed unpaired t-tests were performed, n = 3 biologically independent experiments, ****P < 0.0001. Source data used for the graphs can be found in Supplementary Data 3.

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