Fig. 1
From: Repression of phagocytosis by human CD33 is not conserved with mouse CD33
Expression profile of mCD33 on immune cell subsets. a–i A monoclonal rat IgG1 anti-mouse antibody was used to probe the expression of mCD33 on splenic leukocytes by flow cytometry. For each cell type, a representative histogram (gray: isotype control; black: αmCD33) is shown on the top and a bar graph of the median fluorescence intensity (MFI) for three individual WT (mCD33+/+) and CD33-deficient (mCD33−/−) mice. j Analysis of mCD33 expression on primary microglia isolated directly from an adult brain via a Percoll gradient from three independent WT and mCD33−/− mice. k Levels of mCD33 expression of microglia derived from the brain of neonatal WT (mCD33+/+; black) and CD33-deficient (mCD33−/−; gray) mice. l Levels of mCD33 expression on microglia expanded from the brain of neonatal WT mice cultured with TGF-β1 M-CSF, GM-CSF/ INFγ/LPS, or M-CSF/IL-4/IL-13. Statistical significance calculated based on an unpaired Student’s T-test.
