Fig. 4
From: Repression of phagocytosis by human CD33 is not conserved with mouse CD33
A competitive phagocytic assay to examine cargo uptake in WT and mCD33−/− primary microglia. a Gating strategy for a flow cytometry-based competitive phagocytosis assay in microglia directly isolated from CD45.2+/+ WT or mCD33−/− mice tested in competition verses microglia from CD45.1+/+ WT mice. b–e Results of the competitive flow cytometry-based uptake of b dextran particles (11 independent experiments), c polystyrene beads (9 independent experiments), d myelin (9 independent experiments), and e aggregated Aβ1-42 (5 independent experiments). Shown are representative flow cytometry histograms for WT (CD45.1+) versus mCD33−/− (CD45.2+) and summary plots for each clone for each genotype plotted as a percentage compared with WT CD45.1+ cells. f, g Flow cytometry-based phagocytosis of polystyrene beads (f) and aggregated Aβ1-42 (g) from WT and mCD33−/− microglia expanded from the brain of neonatal mice polarized with GM-CSF/INFγ/LPS where each point represents a different mouse (n = 8). N.S. represents no statistical significance based on an unpaired Student’s T-test. All error bars represent +/− the standard deviation.
