Fig. 1: NHC gold complexes are potent AHR ligands.
a Heatmap of CYPs expression changes upon MC3 treatment for 1 h and 24 h from DNA microarray data (values are log of fold expression change versus DMF treatment). Expression profile of all members of the CYP 450 family and genes related to AHR-NRF2 signaling pathway. b Expression of CYPs and AHR-NRF2 related genes after MC3 treatment analyzed by RT-qPCR (n ≥ 6). c AHR antagonist resveratrol (Res) significantly represses CYP1A1 expression in HepG2 cells treated with MC3 1 µm or TCDD 10 nm for 1 h. Data were normalized to the TCDD-induced CYP1A1 expression (n = 9). d Comparison of CYP1A1 expression in HepG2 AHRKD and CRISPR/CAS control cells, indicated as Ctrl. (n = 9). e Comparison of CYP1A1 and AHR expression in HepG2 control and AHRKD cells treated with TCDD 10 nm or MC3 1 µm for 24 h. One-way ANOVA t test was performed. *p < 0.05, **p < 0.01, ***p < 0.001;. lower and upper ends of the bars indicate the minimum and maximum values, respectively, and the centre represents the median. Error bars ± SD. The source data for 1b–d are provided as Supplementary Data 1.
