Fig. 2: Characterization of free IRDye800CW after IVT dosing in cynomolgus monkeys.

a Profile curves of free IRDye800CW at different doses and at different time points after IVT dosing using the 30° lens and at 60% sensitivity (each dose was tested in one eye). b Representative in vivo images of free IRDye800CW at different time points and diopters after IVT dosing using the 102° lens (60% sensitivity). NA image not available. Scale bars represent 5 mm. c Distribution profiles of free IRDye800CW per animal (n = 2) and averaged at the retina (top) and in the vitreous (bottom) using the 102° lens (60% sensitivity). For these calculations, the retina is defined in these studies as the section at 0 diopter. For the vitreous calculations, a global average across seven sections is presented (16–96 diopters). Control PBS is represented as the dotted line, which delimits the background noise area (in gray) while baseline (3 eyes at the retina and 4 eyes at 21 diopters in the vitreous) is represented as green. Error bars represent the standard deviation between the diopters in the vitreous for each individual curve. d Mean fluorescence of free IRDye800CW across diopters at each day using the 102° lens (60% sensitivity) for n = 2 animals. At Day 3, images at high diopters were not acquired.