Fig. 3: HDX-MS reveals HDL-induced protection on LCAT.

a The panel shows the differences in relative deuterium uptake between LCAT alone and LCAT bound to HDL for all peptides identified and followed by HDX-MS. The exchange time is indicated at the bottom of the panel, increasing from 10 s to 30 min from left to right. All differences are shown in Da and are color-coded according to the scale at the bottom right. Down the left side are the residue numbers of each peptide fragment, arranged from N to C terminus (top to bottom). Peptides highlighted in red are representative of the differences in relative deuterium incorporation and were plotted onto b an open X-ray crystal structure of LCAT (PDB entry 6MVD) at the 10 min timepoint using PyMOL. c Representative deuterium uptake curves for LCAT peptides that show examples of low/no change in deuterium incorporation, and decreased exchange due to protection from HDL binding. All of the deuterium incorporation curves used to create this figure are also shown in Supplementary Fig. 10.