Fig. 5: Fluorescence per cell after 6 h of growth, comparing calibrated flow cytometry to estimates using cell count from CFU and microsphere dilution protocols (LUDOX/water is not shown as the units it produces are not comparable). | Communications Biology

Fig. 5: Fluorescence per cell after 6 h of growth, comparing calibrated flow cytometry to estimates using cell count from CFU and microsphere dilution protocols (LUDOX/water is not shown as the units it produces are not comparable).

From: Robust estimation of bacterial cell count from optical density

Fig. 5: Fluorescence per cell after 6 h of growth, comparing calibrated flow cytometry to estimates using cell count from CFU and microsphere dilution protocols (LUDOX/water is not shown as the units it produces are not comparable).The alternative text for this image may have been generated using AI.

Microsphere dilution produces values extremely close to the ground truth provided by calibrated flow cytometry, whereas the CFU protocol produces values more than an order of magnitude different, suggesting that CFU calibration greatly underestimates the number of cells in the sample. Bars show geometric mean and standard deviation. Team count per condition provided in Supplementary Data 3.

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