Fig. 1: slnrc4a gain of function mutant presents enhanced resistance to diverse plant pathogens and pests.
WT (cv M82) and slnrc4a plants were challenged with several pathogens and pests. Bacterial growth (CFU) was measured 3 days after inoculation with (a) X. campestris (10X5 CFU/mL) and (b) P. syringae (10X5 CFU/mL). c Representative images of P. syringae symptomatic leaves. The lesion area was measured 3 days after inoculation with (d) B. cinerea (10X6 spores/mL) (e) S. sclerotium (10X6 spores/mL). f Representative images of S. sclerotium symptomatic leaves. Infestation was determined by counting number of insects per leaf and measuring % of infected leaf area two-weeks after (g) B. tabaci and (h) T. absoluta exposure, respectively. i Representative images of T. absoluta symptomatic leaves. j O. neolycopersici infection was measured as percentage of infected leaf out of total leaf area. k Representative images of O. neolycopersici symptomatic leaves. Average ± SEM of 3–4 independent replicates is shown. a, b, d, e, g, h, j Boxplots are shown with the inter-quartile-ranges (box), medians (black line in box) and outer quartile whiskers, minimum to maximum values. Asterisks represent statistical significance in t-test with welch correction (*p-value <0.05; **p-value < 0.01; ***p-value < 0.001).
