Fig. 4: Defense gene induction is similar in M82 and slnrc4a in response to MAMP elicitation and pathogen inoculation.

a Gene expression analysis of pathogen responsive genes was measured by RT-qPCR in M82 and slnrc4a plants 24 h after petiole application of EIX. Relative expression normalized to control M82 (mock). Average ± SEM of three independent experiments is shown. No statistically significant differences were observed among WT and slnrc4a (t-test, welch correction). b Gene expression analysis of pathogen responsive genes was measured by RT-qPCR in M82 and slnrc4a samples 24 h after Bc inoculation. Relative expression normalized to M82 (mock). Average ± SEM of three independent experiments is shown. Asterisks represent statistical significance in t-test with welch correction comparing each gene (*p-value < 0.05; ***p-value < 0.001). Boxplots are shown with inter-quartile-ranges (box), medians (black line in box) and outer quartile whiskers, minimum to maximum values.