Fig. 3: Mixed culture of cells carrying a redundant assembly of 11,520 oligos for large-scale data storage.

a Schematic of cells carrying an assembled pool of 11520 oligos for successive multiple subculture. Cells collected from 1^st and 5^th passage were subjected to oligo retrieval and information decoding. b The frequency for each of the 11520 oligos quantified in sequencing reads from the 1^st (blue dots) and 5^th (red dots) passage of the one fragment (1F) assembly sample. c The oligo dropout rate was quantified at different sequencing depths (various amounts of NGS sequencing reads) of the 1^st (blue dots) and 5^th (red dots) passage of the one fragment (1F) assembly sample. d The frequency for each of 11,520 oligos quantified in sequencing reads from the first culture passage of the one insert fragment assembly (1F, blue dots) and three insert fragment assembly (3F, red dots). e The Gini coefficient was calculated for the oligo frequency distribution in the retrieved oligo pool. The 1^st passage of one fragment assembly was calculated to have a Gini of 0.41 (blue line) and the 1^st passage of the three fragments assembly (red line) had a Gini of 0.87.