Fig. 4: Effect of guide RNAs and Cas13b on the expression of a CHIKV split replication system. | Communications Biology

Fig. 4: Effect of guide RNAs and Cas13b on the expression of a CHIKV split replication system.

From: Cas13b-dependent and Cas13b-independent RNA knockdown of viral sequences in mosquito cells following guide RNA expression

Fig. 4

a Aag2, b AF05, c Dcr2 knockout AF319 and d C6/36 cells were co-transfected with plasmid expressing Cas13b (+ Cas13b) or ZsGreen (− Cas13b) and U6 promoter-driven RNA guides, together with a split replication system with nanoluciferase under the control of a CHIKV subgenomic promoter, and a plasmid expressing firefly luciferase as control for transfection efficiency. Each bar represents the mean values of nanoluciferase activity normalised to the firefly luciferase activity (NL/FL), the whiskers are the standard deviation from three independent transfections with six replicates each and each dot represents a replicate. a, b, d Linear mixed-effect model, c two-way ANOVA as model could not be fitted. ***P < 0.001, **P < 0.01 (Supplementary Tables 12–15). C: non-targeting control guide (U6-AmC3), U6-1: guide 1, U6-2: guide 2.

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