Table 3 Productivities and Y(H2/S) of the mono-cultures and consortium grown on glucose and cellobiosea.

From: Biohydrogen production beyond the Thauer limit by precision design of artificial microbial consortia

Glucose

Time [h]

Y(CO2/S) [mol C-mol−1]

Y(H2/S) [mol C-mol−1]

HER [mmol L−1 h−1]

qH2 [mmol h−1 g−1]

CER [mmol L−1 h−1]

E. aerogenes

0–18

     

0–23.5

     

0–40.5

0.03 ± 0.003

0.02 ± 0.002

0.43 ± 0.14

 

0.61 ± 0.13

40.5–45

0.23 ± 0.06

0.14 ± 0.04

0.66 ± 0.32

11.34 ± 0.65

1.18 ± 0.46

45–63

0.10 ± 0.03

0.05 ± 0.02

0.19 ± 0.05

 

0.35 ± 0.08

C. acetobutylicum

0–21

     

0–38.5

     

0–45

     

45–62

0.17 ± 0.03

0.30 ± 0.06

8.86 ± 0.60

86.41 ± 4.51

5.03 ± 0.7

62–86.5

0.46 ± 0.17

0.33 ± 0.12

1.96 ± 0.84

 

2.75 ± 0.50

Consortium

0–16.0

0.09 ± 0.01

0.12 ± 0.05

0.05 ± 0.01

 

0.06 ± 0.01

16–20.0

0.08 ± 0.05

0.05 ± 0.03

0.17 ± 0.02

68.54 ± 5.4

0.27 ± 0.01

20–34.5

0.09 ± 0.01

0.04 ± 0.01

1.73 ± 0.5

9.60 ± 3.2

4.15 ± 0.5

34.5–39.5

0.43 ± 0.02

0.44 ± 0.0.1

6.64 ± 0.25

213.98 ± 8.8

16.19 ± 0.4

39.5–53.0

0.67 ± 0.28

0.93 ± 0.29

4.04 ± 0.22

 

5.84 ± 0.21

Cellobiose

Time [h]

Y(CO2/S) [mol C-mol−1]

Y(H2/S) [mol C-mol−1]

HER [mmol L−1 h−1]

qH2 [mmol h−1 g−1]

CER [mmol L−1 h−1]

E. aerogenes

0–17.5

     

0–22.5

     

0–40

0.08 ± 0.01

0.04 ± 0.003

1.07 ± 0.05

4.68 ± 0.91

2.44 ± 0.23

40–43

0.09 ± 0.01

0.02 ± 0.002

2.00 ± 0.3

0.88 ± 0.71

9.35 ± 0.61

43–61

0.18 ± 0.02

0.04 ± 0.004

0.67 ± 0.06

 

2.96 ± 0.19

61–64

0.06 ± 0.01

0.01 ± 0.002

0.78 ± 0.03

 

3.30 ± 0.24

C. acetobutylicum

0–23

     

0–28

0.01 ± 0.002

0.01 ± 0.002

0.11 ± 0.04

83.43 ± 4.31

0.10 ± 0.03

28–42.5

0.14 ± 0.001

0.25 ± 0.002

9.57 ± 0.13

55.78 ± 9.74

5.43 ± 0.10

42.5–46.5

0.38 ± 0.01

0.43 ± 0.01

5.32 ± 0.31

 

7.64 ± 0.25

46.5–64.5

0.41 ± 0.02

0.27 ± 0.03

1.83 ± 0.12

 

2.80 ± 0.13

Consortium

0–17

     

0–22.5

0.01 ± 0.01

0.06 ± 0.03

0.39 ± 0.01

34.77 ± 3.5

0.06 ± 0.01

22.5–39.5

0.12 ± 0.02

0.36 ± 0.05

10.31 ± 0.22

433.95 ± 10.1

3.32 ± 0.31

39.5–42.5

0.72 ± 0.11

0.73 ± 0.18

7.48 ± 0.16

528.81 ± 8.11

7.30 ± .013

45.5–59.5

0.13 ± 0.06

0.63 ± 0.28

4.42 ± 0.20

 

0.94 ± 0.32

  1. aValues were calculated in between time points (after gas production started) as indicated in the table.