Fig. 2: Selection coefficient graph for ermF by erythromycin.

Selection coefficient values were determined as described previously5. These were plotted with a line of best fit (polynomial regression line order 4, R2 = 0.1709, y = −2.544e−12x4 + 1.564e−09x3 + 2.59e−06x2 − 0.001432x + 0.01684). Here the line of best fit crosses the x-axis at 514.1 µg/L and this is defined as the MSC for this gene selected for by this compound. n = 5 replicates per concentration.