Fig. 1: BAF associates with the centromere. | Communications Biology

Fig. 1: BAF associates with the centromere.

From: A fraction of barrier-to-autointegration factor (BAF) associates with centromeres and controls mitosis progression

Fig. 1

a The patterns of immunolocalization with αBAF antibodies (green), and αHP1a (left), αHP1c (center), and αCENP-C (right) antibodies (red) are presented in interphase S2 cells. DNA was stained with DAPI. Scale bars correspond to 5 μm. b The patterns of immunolocalization with αBAF (green) and αCENP-C (red) antibodies are presented in metaphase chromosomes from S2 cells. Enlarged images are presented in the bottom. Arrows indicate αBAF signals that overlap with αCENP-C signals at centromeres. DNA was stained with DAPI. Scale bars correspond to 2.5 μm. c Immunostainings with αBAF (red) and αCENP-C (green) antibodies are presented in extended chromatin fibers prepared from S2 cells. Scale bar corresponds to 5 μm. d BAF/CENP-C co-immunoprecipitation. IPs were performed with αBAF or αCENP-C antibodies (lanes 3) and control preimmune serum (MOCK) (lanes 2) using S2 cells extracts. IP-materials were analyzed by WB using αCENP-C and αBAF antibodies. Lanes 1 correspond to 3% of the input material. The position of MW markers (in kDa) is indicated.

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