Fig. 6: cenBAF regulates mitosis progression. | Communications Biology

Fig. 6: cenBAF regulates mitosis progression.

From: A fraction of barrier-to-autointegration factor (BAF) associates with centromeres and controls mitosis progression

Fig. 6

a In vivo time-lapse recordings of control dsRNALacZ (top) and dsRNACENP-C (bottom) Nup-107::mRFP cells expressing GFP::CENP-CΔFIMR. mRFP (red) and GFP (green) signals are direct fluorescence. NEBD and NER are indicated. Times are minutes before/after NEBD. Scale bars correspond to 5 μm. b Quantitative analysis of the results shown in a. The total duration of mitosis (left), and the times from NEBD to anaphase onset (AO) (center) and from AO to NER (right) are presented for control dsRNALacZ and dsRNACENP-C Nup-107::mRFP cells expressing the indicated constructs (n = 3; N > 5; Kruskal–Wallis test, *p < 0.05). c Immunostainings with αLaminB antibodies (magenta) and αPS10 (red) of control dsRNALacZ and CENP-C-depleted dsRNACENP-C cells expressing GFP::CENP-CΔFIMR. Mitotic phases are indicated. GFP (green) is direct fluorescence. Scale bars correspond to 5 μm. d The effect of CENP-C depletion on the proportion of mitoses showing NE-assembled αLaminB immunostaining is shown with respect to control dsRNALacZ cells for GFP::CENP-CR and GFP::CENP-CΔFIMR-expressing cells. Values are the sum of 5–8 independent experiments showing equivalent results (N > 92; Chi-square test, ***p < 0.001).

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