Fig. 7: Disrupting cenBAF localization causes NE morphology defects. | Communications Biology

Fig. 7: Disrupting cenBAF localization causes NE morphology defects.

From: A fraction of barrier-to-autointegration factor (BAF) associates with centromeres and controls mitosis progression

Fig. 7

a Immunostainings with αLaminB antibodies (magenta) of control dsRNALacZ and CENP-C-depleted dsRNACENP-C cells expressing the indicated constructs. Scale bars correspond to 10 μm. b Quantitative analysis of the results are shown in a. The percentage of cells showing altered nuclear morphology is presented for control dsRNALacZ and dsRNACENP-C cells expressing the indicated constructs. Values are the sum of 3–4 independent experiments showing equivalent results (N > 334; two-tailed Fischer’s test, ***p < 0.001, ****p < 0.0001). c Enlarged images of immunostainings with αLaminB antibodies (magenta) of dsRNACENP-C cells (images 3–7) and control dsRNALacZ cells (images 1 and 2) expressing GFP::CENP-CΔFIMR. GFP signals are direct fluorescence. Scale bar corresponds to 5 μm.

Back to article page