Fig. 8: cenBAF, PP4, and CENP-C are interdependent for centromeric localization.
a Schematic representation of the interdependences for centromeric localization between cenBAF, PP4, and CENP-C. CENP-C mediates centromeric recruitment of PP4, which is required to retain cenBAF at centromeres during mitosis. cenBAF stabilizes CENP-C at centromeres. b Immunostainings with αFlfl (green) and αCENP-C antibodies (red) are presented for mitotic chromosomes from dsRNAFlfl (right) and control dsRNALacZ (left) cells. DNA was stained with DAPI. Arrows in dsRNAFlfl cells indicate centromeric αCENP-C signals. Scale bar corresponds to 5 μm. c Immunostainings with αFlfl (green) and αCENP-C antibodies (red) are presented for mitotic chromosomes from dsRNABAF (right) and control dsRNALacZ (left) cells. DNA was stained with DAPI. Arrows indicate αFlfl signals in dsRNABAF cells overlapping with αCENP-C at centromeres. Scale bar corresponds to 5 μm. d Immunostainings with αFlfl (green) and αCENP-C antibodies (red) are presented for mitotic chromosomes from dsRNACENP-C (right) and control dsRNALacZ (left) cells. DNA was stained with DAPI. Scale bar corresponds to 5 μm. e Immunostainings with αBAF (green) and αCENP-C antibodies (red) are presented for mitotic chromosomes from dsRNACENP-C (right) and control dsRNALacZ (left) cells. DNA was stained with DAPI. Scale bar corresponds to 5 μm.
