Fig. 3: Transition rate and free energy barriers.
From: DNA minor-groove binder Hoechst 33258 destabilizes base-pairing adjacent to its binding site
a Average observed rate (λobs) calculated from the amplitude-weighted mean across the maximum. Error bars represent the standard deviation of the averaged rates. b Calculated association (ka) and dissociation (kd) rate constants of DNA (circles) and H-DNA (triangles) and their fits from Eq. (2). *The unit of ka is M−1 s−1 in the DNA association, and M−2 s−1 in the H-DNA association. c, d Free energy barriers of association (\({\Delta}G_{\mathrm{a}}^\dagger\)) and dissociation (\({\Delta}G_{\mathrm{d}}^\dagger\)) of DNA and H-DNA.
