Table 1 Thermodynamics parameters for melting transition.

From: DNA minor-groove binder Hoechst 33258 destabilizes base-pairing adjacent to its binding site

Sample

CD (M)

Tm (oC)a

T0 (oC)b

\({\Delta}H_{\mathrm{d}}^0\) (kJ mol−1)

\({\Delta}S_{\mathrm{d}}^0\) (J mol−1 K−1)

DNA

1 × 10−3

77

97

403

1088

H-DNA

1 × 10−3

93

142

415

1000

2 × 10−6

63

   

2 × 10−7

52

   
  1. aTm is defined as the temperature at which the DNA duplex fraction is 0.5.
  2. bT0 is defined as the temperature where Kd = 1 M and \({\Delta}G_{\mathrm{d}}^0\) = 0.
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