Fig. 2: Effects of MAPK or PI3K/mTOR inhibition on IL-8 expression. | Communications Biology

Fig. 2: Effects of MAPK or PI3K/mTOR inhibition on IL-8 expression.

From: BRAF status modulates Interelukin-8 expression through a CHOP-dependent mechanism in colorectal cancer

Fig. 2

SNU1235, SNU1047, HT29, and LS180 cell lines were treated with increasing concentration of drugs (dose range 0.01–1 μM dabrafenib (D), 1–100 nM trametinib (T) and 1–100 nM gedatolisib (G)) (a, b) or with D in combination with T at fixed doses (c), as indicated. IL-8 expression was measured after 24 h of treatment, by IL-8 ELISA assay; the percentage of IL-8 was obtained from pg/mL assuming the levels in control cells as 100%. Results represent the average of three independent experiments. For statistical analysis, ANOVA (a, b) or 2-tailed Student’s t test for the comparison between control and treated samples (c) were used as appropriate.

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