Table 1 Genetic characterization of 28 CRC cell lines.

Cell line	BRAF	KRAS	PTEN			PI3K
Cell line	BRAF	KRAS	Gene	mRNA abundance^a	Protein expression^b	PI3K
SNU1544	wt	wt	wt	0.21	+	p.H1047R
MDST8	p.V600K	wt	wt	0.00	−	wt
OUMS23	p.V600E	wt	n.a.	0.01	−	wt
LIM2537	p.V600E	wt	wt	0.20	−	wt
LIM2412	p.V600E	wt	wt	0.13	−	wt
LS411N	p.V600E	wt	p.C105fs*	0.10	−	wt
SNUC4	wt	wt	p.F241S; V290*	0.62	−	p.V71I; p.E545G
SNUC5	p.V600E	wt	wt	0.78	+	p.H1047R
SNU1040	wt	wt	p.R335*; p.T232A	0.00	−	wt
SNU1047	wt	wt	p.K267fs	0.15	−	wt
SNU1235	p.V600E	wt	p.R130*	0.11	−	wt
KM12C	wt	wt	p.G129*	0.19	−	wt
VACO432	p.V600E	wt	wt	0.32	++	p.H1047R
HT29	p.V600E	wt	wt	0.54	++	wt
LS180	wt	p.G12D	wt	0.46	++	p.H1047R
SW620	wt	p.G12V	wt	0.39	++	wt
SW480	wt	p.G12V	wt	0.12	+	wt
COLO205	p.V600E	wt	wt	0.37	++	wt
RKO	p.V600E	wt	wt	0.11	+	p.I391M; p.H1047R
COGA3	wt	p.G13D	p.R173H	0.30	−	wt
HROC87	p.V600E	wt	n.a.	0.29	++	wt
HCT116	wt	p.G13D/wt	wt	0.21	+	p.H1047R
HCT116 PTEN^−/−	wt	p.G13D	del ex5	0.11	−	p.H1047R
HKE-3	wt	wt	wt	0.36	+	p.H1047R
HK2–6	wt	p.G12C; p.G13D/G13D	wt	0.21	+	p.H1047R
DLD-1	wt	p.G13D/wt	wt	0.38	++	p.E545K; p.D549N
DKO-1	wt	p.G12C; p.G13D/G13D	wt	0.33	++	p.E545K; p.D549N
DKO-4	wt	wt	wt	0.36	++	p.E545K; p.D549N

^aResults represent PTEN mRNA abundance relative to positive control T98G
^bOD ratio of PTEN antibody/β-Actin for each individual sample is compared with OD of positive control T98G. + score 0.01–0.59; + + score 0.6–1. n.a., not available
*Indicates a stop codon

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