Fig. 4: Changes to α-chymotrypsin FTIR spectra with pressure.
From: High pressures increase α-chymotrypsin enzyme activity under perchlorate stress
Normalised pressure-dependent FTIR spectra of α-CT (50 mg mL−1) in (a) neat buffer (+10 mM CaCl2) (a), (c) 0.25 M Mg(ClO4)2 and (e) at 35 °C, 0.5 M Mg(ClO4)2 at 25 °C, and the corresponding secondary structural changes (b, d, f) obtained from the curve fitting procedure. Arrows indicate increasing pressure and lines show the Boltzmann fits to the experimental data using Eq. (4).
